How does high-performance liquid chromatography (HPLC) separate components in a mixture?
- By boiling point
- By charge-to-size ratio
- By size exclusion
- By partitioning between a stationary phase and a mobile phase
Explanation
High-performance liquid chromatography (HPLC) separates components in a mixture by partitioning between a stationary phase and a mobile phase.
Partitioning:
In HPLC, the sample mixture is introduced into a column packed with a stationary phase (a solid material) and a liquid mobile phase flows through the column.
Differential retention:
Different components in the mixture have different affinities for the stationary phase and the mobile phase.
Separation:
As the mobile phase flows through the column, the components with a stronger affinity for the stationary phase will spend more time interacting with it,
Resulting in slower movement through the column, while the components with a weaker affinity will move faster
Related MCQs
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- Transition metal
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اس سوال کو وضاحت کے ساتھ پڑھیں
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- Calcium
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- C₆H₆
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- None of these
اس سوال کو وضاحت کے ساتھ پڑھیں
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- Chemical properties
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اس سوال کو وضاحت کے ساتھ پڑھیں
سب سے سادہ الکین کون سا ہے؟
- Propane
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- None of these
اس سوال کو وضاحت کے ساتھ پڑھیں
